Review



lc3 proteintech  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Proteintech lc3 proteintech
    Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for <t>LC3B</t> (green). Quantification of the mean fluorescence intensity of <t>LC3</t> is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001
    Lc3 Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1830 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc3 proteintech/product/Proteintech
    Average 96 stars, based on 1830 article reviews
    lc3 proteintech - by Bioz Stars, 2026-05
    96/100 stars

    Images

    1) Product Images from "S100A11 regulates microglial inflammatory response in neuropathic pain via H3K27ac-TFEB-mitochondrial autophagy axis"

    Article Title: S100A11 regulates microglial inflammatory response in neuropathic pain via H3K27ac-TFEB-mitochondrial autophagy axis

    Journal: The Journal of Headache and Pain

    doi: 10.1186/s10194-026-02328-9

    Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for LC3B (green). Quantification of the mean fluorescence intensity of LC3 is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001
    Figure Legend Snippet: Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for LC3B (green). Quantification of the mean fluorescence intensity of LC3 is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001

    Techniques Used: Knockdown, Western Blot, Labeling, Immunofluorescence, Staining, Fluorescence

    S100A11 knockdown alleviates microglial inflammation by inhibiting mitophagy through the TFEB/H3K27ac axis. A-B Western blot analysis of TFEB, TFEC, and TFE3 protein expression levels in the cytoplasm and nucleus of primary microglia, along with the corresponding detection and quantification plots ( D-F , for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). C Immunofluorescence staining of TFEB in primary microglia. G Western blot analysis of pink1, Parkin, P62, LC3II protein levels of primary microglia; H-K Relative levels normalized to COX IV were quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). M Labeling of mitochondria (red) in primary microglia with Tom20; immunofluorescence staining for LC3B (green). Quantification of the mean fluorescence intensity of LC3 is shown in panel L . and relative fluorescence intensity was quantified in panel L ( n = 3). N Western blot analysis of H4K16ac, H4K12ac, H3K27ac, H3K18ac, H3K9ac protein levels of microglia; O-S Relative levels normalized to histone H3 were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). T Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; U-X Relative levels normalized to COX IV were quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Z Labeling of mitochondria (red) in primary microglia with Tom20; immunofluorescence staining for LC3B (green), and relative fluorescence intensity was quantified in panel Y ( n = 3). Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001
    Figure Legend Snippet: S100A11 knockdown alleviates microglial inflammation by inhibiting mitophagy through the TFEB/H3K27ac axis. A-B Western blot analysis of TFEB, TFEC, and TFE3 protein expression levels in the cytoplasm and nucleus of primary microglia, along with the corresponding detection and quantification plots ( D-F , for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). C Immunofluorescence staining of TFEB in primary microglia. G Western blot analysis of pink1, Parkin, P62, LC3II protein levels of primary microglia; H-K Relative levels normalized to COX IV were quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). M Labeling of mitochondria (red) in primary microglia with Tom20; immunofluorescence staining for LC3B (green). Quantification of the mean fluorescence intensity of LC3 is shown in panel L . and relative fluorescence intensity was quantified in panel L ( n = 3). N Western blot analysis of H4K16ac, H4K12ac, H3K27ac, H3K18ac, H3K9ac protein levels of microglia; O-S Relative levels normalized to histone H3 were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). T Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; U-X Relative levels normalized to COX IV were quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Z Labeling of mitochondria (red) in primary microglia with Tom20; immunofluorescence staining for LC3B (green), and relative fluorescence intensity was quantified in panel Y ( n = 3). Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001

    Techniques Used: Knockdown, Western Blot, Expressing, Immunofluorescence, Staining, Labeling, Fluorescence



    Similar Products

    lc3 proteintech  (Proteintech)
    96
    Proteintech lc3 proteintech
    Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for <t>LC3B</t> (green). Quantification of the mean fluorescence intensity of <t>LC3</t> is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001
    Lc3 Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc3 proteintech/product/Proteintech
    Average 96 stars, based on 1 article reviews
    lc3 proteintech - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier
    lc3 14600 1 ap proteintech wuhan china  (Proteintech)
    96
    Proteintech lc3 14600 1 ap proteintech wuhan china
    Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for <t>LC3B</t> (green). Quantification of the mean fluorescence intensity of <t>LC3</t> is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001
    Lc3 14600 1 Ap Proteintech Wuhan China, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc3 14600 1 ap proteintech wuhan china/product/Proteintech
    Average 96 stars, based on 1 article reviews
    lc3 14600 1 ap proteintech wuhan china - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier
    rabbit anti lc3b polyclonal antibody proteintech  (Proteintech)
    96
    Proteintech rabbit anti lc3b polyclonal antibody proteintech
    Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for <t>LC3B</t> (green). Quantification of the mean fluorescence intensity of <t>LC3</t> is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001
    Rabbit Anti Lc3b Polyclonal Antibody Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti lc3b polyclonal antibody proteintech/product/Proteintech
    Average 96 stars, based on 1 article reviews
    rabbit anti lc3b polyclonal antibody proteintech - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier
    1 ap proteintech  (Proteintech)
    96
    Proteintech 1 ap proteintech
    Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for <t>LC3B</t> (green). Quantification of the mean fluorescence intensity of <t>LC3</t> is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001
    1 Ap Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 ap proteintech/product/Proteintech
    Average 96 stars, based on 1 article reviews
    1 ap proteintech - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier
    ratio loading amount lc3 14600 1 ap proteintech  (Proteintech)
    96
    Proteintech ratio loading amount lc3 14600 1 ap proteintech
    Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for <t>LC3B</t> (green). Quantification of the mean fluorescence intensity of <t>LC3</t> is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001
    Ratio Loading Amount Lc3 14600 1 Ap Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ratio loading amount lc3 14600 1 ap proteintech/product/Proteintech
    Average 96 stars, based on 1 article reviews
    ratio loading amount lc3 14600 1 ap proteintech - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier
    lc3 proteintech 14600 1 ap  (Proteintech)
    96
    Proteintech lc3 proteintech 14600 1 ap
    Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for <t>LC3B</t> (green). Quantification of the mean fluorescence intensity of <t>LC3</t> is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001
    Lc3 Proteintech 14600 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc3 proteintech 14600 1 ap/product/Proteintech
    Average 96 stars, based on 1 article reviews
    lc3 proteintech 14600 1 ap - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier
    1 ap proteintech ab 2137737  (Proteintech)
    96
    Proteintech 1 ap proteintech ab 2137737
    Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for <t>LC3B</t> (green). Quantification of the mean fluorescence intensity of <t>LC3</t> is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001
    1 Ap Proteintech Ab 2137737, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 ap proteintech ab 2137737/product/Proteintech
    Average 96 stars, based on 1 article reviews
    1 ap proteintech ab 2137737 - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for LC3B (green). Quantification of the mean fluorescence intensity of LC3 is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001

    Journal: The Journal of Headache and Pain

    Article Title: S100A11 regulates microglial inflammatory response in neuropathic pain via H3K27ac-TFEB-mitochondrial autophagy axis

    doi: 10.1186/s10194-026-02328-9

    Figure Lengend Snippet: Knockdown of S100A11 improves inflammatory response and pyroptosis in primary microglia by inhibiting mitophagy. A Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; B-E Relative levels normalized to COXIV were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). G-H Labeling of mitochondria in primary microglia with Tom20 (red); immunofluorescence staining for LC3B (green). Quantification of the mean fluorescence intensity of LC3 is shown in panels F and I . (Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Immunofluorescence staining of CD68 ( J ) and CD206 ( K ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels. L , M ( Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with appropriate post hoc tests), for three independent experiments. Q Western blot analysis of NLRP3 protein levels of microglia; N Relative levels normalized to β-actin was quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Immunofluorescence staining of NLRP3 ( R ) and Caspase1 ( S ) in microglia (red); green fluorescence indicates phalloidin. Quantification of relative fluorescence intensities is shown in panels O , P (Statistical significance was determined by one-way ANOVA with appropriate post hoc tests) for three independent experiments. Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001

    Article Snippet: Membranes were blocked with 5% skim milk for 60 min, rinsed with TBST, and incubated with primary antibodies at 4 °C overnight (β-actin: Proteintech, 66009-1-Ig, 1:10000; S100A11: Abcam, ab169530, 1:1000; NLRP3: AdipoGen, AG-20B-0014, 1:1000; GSDMD-N: Abcam, ab215203, 1:1000; ASC: Wanleibio, WL02462, 1:500; Caspase1: CST, 89332, 1:1000; Bax: Proteintech, 50599-2-Ig, 1:2000; Bcl-2: abclone, A19693, 1:2000; COXIV: Proteintech, 11242-1-AP, 1:1000; LC3: Proteintech, 14600-1-AP, 1:2000; P62: Proteintech, 18420-1-AP, 1:2000; Parkin: Proteintech, 14060-1-AP, 1:1000; Pink1: Proteintech, 23274-1-AP, 1:2000; H3: Proteintech, 17168-1-AP, 1:2000; TFEB: Proteintech, 13372-1-AP, 1:1000; TFEC: Proteintech, 13547-1-AP, 1:1000; TFE3: Proteintech, 14480-1-AP, 1:1000; H3K9ac: CST, 9649, 1:1000; H3K18ac: CST, 13998, 1:1000; H3K27ac: CST, 8173, 1:1000; H4K12ac; CST, 13944, 1:1000; H4K16ac: CST, 13534, 1:1000).

    Techniques: Knockdown, Western Blot, Labeling, Immunofluorescence, Staining, Fluorescence

    S100A11 knockdown alleviates microglial inflammation by inhibiting mitophagy through the TFEB/H3K27ac axis. A-B Western blot analysis of TFEB, TFEC, and TFE3 protein expression levels in the cytoplasm and nucleus of primary microglia, along with the corresponding detection and quantification plots ( D-F , for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). C Immunofluorescence staining of TFEB in primary microglia. G Western blot analysis of pink1, Parkin, P62, LC3II protein levels of primary microglia; H-K Relative levels normalized to COX IV were quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). M Labeling of mitochondria (red) in primary microglia with Tom20; immunofluorescence staining for LC3B (green). Quantification of the mean fluorescence intensity of LC3 is shown in panel L . and relative fluorescence intensity was quantified in panel L ( n = 3). N Western blot analysis of H4K16ac, H4K12ac, H3K27ac, H3K18ac, H3K9ac protein levels of microglia; O-S Relative levels normalized to histone H3 were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). T Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; U-X Relative levels normalized to COX IV were quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Z Labeling of mitochondria (red) in primary microglia with Tom20; immunofluorescence staining for LC3B (green), and relative fluorescence intensity was quantified in panel Y ( n = 3). Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001

    Journal: The Journal of Headache and Pain

    Article Title: S100A11 regulates microglial inflammatory response in neuropathic pain via H3K27ac-TFEB-mitochondrial autophagy axis

    doi: 10.1186/s10194-026-02328-9

    Figure Lengend Snippet: S100A11 knockdown alleviates microglial inflammation by inhibiting mitophagy through the TFEB/H3K27ac axis. A-B Western blot analysis of TFEB, TFEC, and TFE3 protein expression levels in the cytoplasm and nucleus of primary microglia, along with the corresponding detection and quantification plots ( D-F , for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). C Immunofluorescence staining of TFEB in primary microglia. G Western blot analysis of pink1, Parkin, P62, LC3II protein levels of primary microglia; H-K Relative levels normalized to COX IV were quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). M Labeling of mitochondria (red) in primary microglia with Tom20; immunofluorescence staining for LC3B (green). Quantification of the mean fluorescence intensity of LC3 is shown in panel L . and relative fluorescence intensity was quantified in panel L ( n = 3). N Western blot analysis of H4K16ac, H4K12ac, H3K27ac, H3K18ac, H3K9ac protein levels of microglia; O-S Relative levels normalized to histone H3 were quantified (for three independent experiments, statistical significance was determined by two-way ANOVA with appropriate post hoc tests). T Western blot analysis of Pink1, Parkin, P62, LC3II protein levels of primary microglia; U-X Relative levels normalized to COX IV were quantified (for three independent experiments, statistical significance was determined by one-way ANOVA with appropriate post hoc tests). Z Labeling of mitochondria (red) in primary microglia with Tom20; immunofluorescence staining for LC3B (green), and relative fluorescence intensity was quantified in panel Y ( n = 3). Scale bars as indicated. Data are presented as mean ± SEM. * P < 0.05 , ** P < 0.01 , *** P < 0.001

    Article Snippet: Membranes were blocked with 5% skim milk for 60 min, rinsed with TBST, and incubated with primary antibodies at 4 °C overnight (β-actin: Proteintech, 66009-1-Ig, 1:10000; S100A11: Abcam, ab169530, 1:1000; NLRP3: AdipoGen, AG-20B-0014, 1:1000; GSDMD-N: Abcam, ab215203, 1:1000; ASC: Wanleibio, WL02462, 1:500; Caspase1: CST, 89332, 1:1000; Bax: Proteintech, 50599-2-Ig, 1:2000; Bcl-2: abclone, A19693, 1:2000; COXIV: Proteintech, 11242-1-AP, 1:1000; LC3: Proteintech, 14600-1-AP, 1:2000; P62: Proteintech, 18420-1-AP, 1:2000; Parkin: Proteintech, 14060-1-AP, 1:1000; Pink1: Proteintech, 23274-1-AP, 1:2000; H3: Proteintech, 17168-1-AP, 1:2000; TFEB: Proteintech, 13372-1-AP, 1:1000; TFEC: Proteintech, 13547-1-AP, 1:1000; TFE3: Proteintech, 14480-1-AP, 1:1000; H3K9ac: CST, 9649, 1:1000; H3K18ac: CST, 13998, 1:1000; H3K27ac: CST, 8173, 1:1000; H4K12ac; CST, 13944, 1:1000; H4K16ac: CST, 13534, 1:1000).

    Techniques: Knockdown, Western Blot, Expressing, Immunofluorescence, Staining, Labeling, Fluorescence